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From 1980 to 1995, 3,181 deaths from paracoccidioidomycosis occurred in Brazil, representing the eighth most common cause of death from predominantly chronic or recurrent types of infectious and parasitic diseases, showing considerable magnitude and low visibility. Tropical diseases, such as paracoccidioidomycosis, are the most common type of neglected diseases.

ELISA and western blot can be used as complementary tests. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii-CFA antigen, but sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. In western blot, sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. ELISA was found to be a very useful test to titer anti- P. Non-reactive sera for traditional antigens (n = 17) were tested with different P. brasiliensis using traditional antigens (AgPbB339 and gp43). Seventy-two sera were defined as reactive for P. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. This study aimed to develop new antigenic preparations from P. Serological diagnosis of PCM caused by P. Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil.

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